Plant Bio-technology and Plant Tissue Culture
Tissue culture is the in vitro aseptic culture of cells, tissues, organs or whole plant under controlled nutritional and environmental conditions often to produce the clones of plants. The resultant clones are true-to type of the selected genotype. The controlled conditions provide the culture an environment conducive for their growth and multiplication. These conditions include proper supply of nutrients, pH medium, adequate temperature, and proper gaseous and liquid environment.